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regierungspräsidium karlsruhe brief

regierungspräsidium karlsruhe brief

Thereafter, RNA was isolated with the Arcturus PicoPure RNA Isolation Kit (Life Technologies), and RNA quality and quantity were analyzed on an Agilent Bioanalyzer. To label the proliferating liver ECs after PHx, EdU (40 mg/kg) was administered intraperitoneally daily during the angiogenic phase of liver regeneration (days 2–6). Thus, under nonvascular-damaging conditions, the vascular regeneration proceeds exclusively by the expansion of preexisting tissue-resident ECs. Scale bars, 100 µm. In case of chemotherapy 150 l (0.5 mg/ml) cisplatin were injected intraperitoneally (i.p.) Add photos. 3 G). In brief, inclusion criteria were study enrollment within 24 h after symptom onset. Animals were allowed free access to food and water before starting the experiments. Our data demonstrate that (1) irradiation causes irreversible damage to the liver vasculature, thereby restricting the proliferative capacity of residing liver ECs and resulting in recruitment and incorporation of BMDMCs for vascular repair; (2) under nonvascular-damaging conditions, reconstitution of the liver vasculature relies on preexisting vessels with no direct contribution of BMDMCs; and (3) systemically infused bone marrow cells or G-CSF–mobilized progenitor cells do not integrate into the regenerating liver vasculature after PHx. Hence, it is necessary to use better preclinical liver regeneration models that allow quantitative assessment of BMDMC contribution to the newly formed blood vessels in clinically relevant pathophysiological settings. A longitudinal skin incision was made to expose the elbow and knee joint. In brief, 2.5 μg of nuclear extracts isolated from cell pellets with Nuclear Extract Kit (Active Motif, Inc., Carlsbad, USA) were incubated with a synthetic oligonucleotide containing the c-MYC consensus sequence. Section III: Legal, economic, financial and technical information. The power of new sequencing methods now makes it possible to reinvigorate medaka genetics, in particular by establishing a near-isogenic panel derived from a single wild population. Plates were further incubated for 4 hours at 37 C and the formazan crystals formed were dissolved by adding 110μl/well acidified 2-propanol (0.04 N HCl). Arrows indicate phospho-H2A.X (Ser139)–positive ECs. "lang":"en" (F and G) Quantitative PCR analysis of mRNA expression of Bax (F) and Icam1 (G) in livers of mice after irradiation or PHx (mean ± SD, n = 5–6 mice for each time point). S2, B and C). The animal studies were approved by the responsible authority for animal experiments (Regierungspraesidium Karlsruhe). (B) The ratio of GFP+ ECs in the livers of Para-WT (sham operated and PHx) as well as Para-GFP (sham operated and PHx) mice was analyzed by FACS (mean ± SD, n = 3–4 mice). The mesothelium is a dynamic and specialized tissue layer that covers the somatic cavities (pleural, peritoneal, and pericardial) as well as the surface of the visceral organs such as the lung, heart, liver, bowel and tunica vaginalis testis. After centrifugation, the cells were incubated in staining solution with antibodies CD45-PE (#561087; BD Pharmingen), CD45-APC-Cy7 (#557659; BD Pharmingen), CD31-APC (#551262; BD Pharmingen), CD133-FITC (#11-1331-82; eBioscience), and CD146-PerCP-Vio700 (#130-103-865; Miltenyi Biotec). List and brief description of selection criteria: Nachweis der Eignung durch: ... Regierungspräsidium Karlsruhe, Referat 47.2 – Baureferat Mitte Schlossplatz 4-6 76131 Karlsruhe Baden-Württemberg. Blood samples were taken from the Vena facialis after 4 hours of fasting during the day cycle. All animal experiments were approved by the institutional and governmental Animal Care and Use Committees (IRCBC-2016-02 to J. Hu and G220/11, G213/17, and G219/17 to H.G. Once the ALT/AST levels subsided, mice were euthanized, and livers were collected for FACS analysis or immunostaining. We are shaping the future We are shaping the future. Consistent with a previous report (Höfer et al., 2016), the VECad promoter was specifically active in ECs and silenced in the hematopoietic compartment of adult mice. Further, flow cytometry analysis revealed no significant changes in the frequencies of YFP+ liver ECs between mice treated with either oil or CCl4 (Fig. Save. All animal experiments were approved by the institutional and governmental Animal Care and Use Committees (IRCBC-2016-02 to J. Hu and G220/11, G213/17, and G219/17 to H.G. We develop and publish International Standards. the regional licensing body (“Regierungspräsidium Karlsruhe”) and the specific experiment protocol was approved by the ethics committee attached to Regierungspräsidium Karlsruhe. In summary, using a wide array of lineage-tracing tools, the present study was aimed at unveiling the source of newly formed vessels during liver regeneration. Then, a customized circular lead plate was put over the upper abdomen of the mouse to protect the liver from irradiation. (E) FACS analysis of donor chimerism in CD45+ cells from peripheral blood of Rag2−/−γc−/−KitW/Wv recipients (mean ± SD, n = 4 mice). Two-tailed Student’s t test. 4, D and E). 3 C and Fig. Experimental animals were generated by breeding of hemizygous βENaC-TG mice with C3B6 F1 WT mice (Charles River … We're ISO, the International Organization for Standardization. C57BL/6 Rosa26-YFPfl/fl mice were crossed with C57BL/6 VECad-CreERT2 mice to specifically label ECs upon tamoxifen application. Schuljahresbeginn 2020/2021 - Regierungspräsidium Karlsruhe informiert über Veränderungen bei Schülerzahlen, Unterrichtsversorgung und schulartbezogene Entwicklungen im Regierungsbezirk Karlsruhe. (31571203), Recruitment Program of Global Experts (C) The percentage of GFP+ ECs in livers of mice irradiated in the presence or absence of a liver shield was analyzed by FACS (mean ± SD, n = 6 mice). Cells The C26 (derived from BALB/c Colon26 murine colon adenocarcinoma) and Renca (renal cell carcinoma of BALB/c origin) cell lines were provided by the tumor-bank of the German Cancer Research Center (Heidelberg, Germany) and cultured in DMEM medium with high Glucose-4500 mg/L … The generation of βENaC-TG mice (line 6608) has been described previously . Livers were collected and minced into small pieces and incubated with collagenase I buffer (200 U/ml collagenase I, 0.01% CaCl2, and 1.25% DNase in DMEM) for 30 min at 37°C with agitation. First, VECad-CreERT2xRosa26-YFPfl/fl mice with labeled ECs were injected with either oil or CCl4 to induce liver injury over a period of 4 wk. You can find all important news stories, headlines, news photos and videos about Karlsruhe. We now show in zebrafish that the increase in cortisol elicited by a brief period of involuntary swimming peaks at 6 days post fertilization ... (Regierungspräsidium Karlsruhe; G-29/12). (B) The frequency of YFP+ hematopoietic cells (CD45+) in the liver and the peripheral blood of sham-operated and PHx mice was analyzed by FACS (mean ± SD, n = 4–5 mice). Dort finden Sie neben dem Antragsformular nützliche Informationen zu den Anspruchsvoraussetzungen und zur Antragstellung. Mahak Singhal, Xiaoting Liu, Donato Inverso, Kai Jiang, Jianing Dai, Hao He, Susanne Bartels, Weiping Li, Ashik Ahmed Abdul Pari, Nicolas Gengenbacher, Eva Besemfelder, Lijian Hui, Hellmut G. Augustin, Junhao Hu; Endothelial cell fitness dictates the source of regenerating liver vasculature. Following a recovery period of 2 wk after CCl4 treatment, Sirius red staining was performed on the liver sections of CCl4-treated mice, which showed a strong deposition of collagen, an indication of liver fibrosis (Fig. S. Bartels contributed to the irradiation-primed bone marrow transplantation model. Karlsruhe was an old small ship, but in those days, every unit capable of evacuating people to the west was important. Various genes, up-regulated in atherosclerosis but also by psychosocial stress, are controlled by the transcription factor nuclear factor κB (NF-κB). Bone marrow cells of PanRosaYFP mice were flushed from femurs, tibias, coxae, and humeri using PBS supplemented with 5% heat-inactivated FCS. S1, F and G), indicating that both cell populations possess a similar proliferation capacity. (A) Experimental outline of PHx-induced liver regeneration in C57BL/6N mice. Not Now. Circulating YFP+ cells could infiltrate into the liver tissue, as they constituted around 25% of the CD45+ population in the livers of BM-transplanted NSG mice (Fig. Quantitative PCR analysis revealed higher expression of Bax, an apoptotic activator, in livers of irradiated but not partially hepatectomized mice compared with nonirradiated and partially hepatectomized mice (Fig. 89077 Ulm Tel 0731 185-1640 Mail Stadtkreis Baden-Baden … Yet, when comparing the livers of PHx and sham-operated animals, there was no significant incorporation of YFP+ cells among total liver ECs (Fig. FORSTBW MANAGEMENT Headquarter Ministry of Rural Affairs and Consumer Protection Kernerplatz 10 . The cell suspension was further treated with 5 ml 1× ACK lysis buffer to remove erythrocytes. — um die Namen der Nachunternehmer ergänztes Nachunternehmervereichnis. Thus, our results demonstrate that irradiation induced EC double-strand DNA breaks and apoptosis, which eventually led to impaired endothelial self-repair. The animal studies were approved by the responsible authority for animal experiments (Regierungspraesidium Karlsruhe). After hearing B, the Regierungspräsidium Karlsruhe (Regional Council, Karlsruhe, Germany) determined, by decision of 25 November 2014, adopted on the basis of Paragraph 6(5) of the FreizügG/EU in conjunction with Article 28(3)(a) of Directive 2004/38, that B had lost his right of entry to and residence in Germany. After washing, protein–DNA complexes were eluted and cross-links were reversed. — 4 x Wasserhaltung inkl. Precise information on deadline(s) for review procedures: Official name: Regierungspräsidium Karlsruhe, Referat 41, Supplement to the Official Journal of the EU, Duration of the contract, framework agreement or dynamic purchasing system, Suitability to pursue the professional activity, including requirements relating to enrolment on professional or trade registers, Information about a framework agreement or a dynamic purchasing system, Information about the Government Procurement Agreement (GPA), Time limit for receipt of tenders or requests to participate, Estimated date of dispatch of invitations to tender or to participate to selected candidates. In brief, the EcoRI-BamHI fragment coding for tTA2s (from pUHT61-1 ) and the EcoRI CreERT2 fragment ... (Regierungspräsidium Karlsruhe) and carried out in accordance with the local Animal Welfare Act and the European Communities Council Directive of 24 November 1986 (86/609/EEC). Dass sich das Unternehmen bei der Berufsgenossenschaft angemeldet hat. Two-tailed Student’s t test. Das … Community See All. In line with a previous report (Verbiest et al., 2016), the NSG mice successfully accepted the allograft, as there were ∼15% YFP+ cells among total circulating CD45+ cells in the peripheral blood on day 10 following PHx (Fig. All animal experiments were approved by the local animal ethics committee (Regierungspräsidium Karlsruhe; Ministerium für Landwirtschaft, Umwelt und ländliche Räume, Kiel, Germany). Bisected kidney basins were used as enrichment. Official name: Regierungspräsidium Karlsruhe, Referat 47.2 – Baureferat Mitte Postal address: Schlossplatz 4-6 Town: Karlsruhe NUTS code: DE122 Karlsruhe Postal code: 76131 Country: Germany E-mail: Telephone: +49 721926-0 Fax: +49 72193340240 Internet address(es): Main address: For Video 1, a semiautomatic surface rendering module in Imaris (Bitplane) was used to create three-dimensional volumetric surface objects corresponding either to individual cells or to the liver vascular system. To fuse the blood circulation of two independent mice, female WT and CAG-GFP mice were subjected to parabiotic surgery as described previously (Kamran et al., 2013). Afterwards, mice were subjected to two-thirds PHx. (G) Experimental outline of the VECad-CreERT2xRosa26-YFPfl/fl genetic labeling model. Volunteers were subjected to a brief laboratory stress test and NF-κB activity was … Augustin); the European Research Council Advanced Grant “AngioMature” (787181 to H.G. "link" : "", Most of the GFP+ cells in the livers of Para-WT-PHx mice were CD45+ and maintained their hematopoietic identity (Fig. The colony was originally established and maintained on a mixed genetic background (C3H/HeN × C57BL/6N). Cells were resuspended with PBS and analyzed by flow cytometry. To investigate why irradiation-based conditioning induced BMDMC incorporation into the liver vasculature, WT mice were irradiated with 9 Gy. Tenders or requests to participate must be submitted to the following address: The procurement is related to a project and/or programme financed by European Union funds: no. M. Singhal and X. Liu contributed equally to this work. Two mouse lungs of similar genotype were pooled, minced, and digested with collagenase A solution (Roche). In brief, magnetic beads (Dynabeads; Dynal) were coated with anti-CD31 antibodies (Mec13.3; BD Biosciences). Sections were then incubated with primary antibodies overnight at 4°C, followed by fluorophore-conjugated secondary antibodies for 2 h at room temperature. / FH / … Augustin and J. Hu contributed equally to this work. Both expression systems allow for spatial and temporal control of gene activities, and, in the case of … Donor chimerism of blood cells was determined 1 mo after transplantation. A microarray analysis comparing the bone marrow–recruited ECs and the corresponding resident liver ECs revealed that BMDMCs incorporated as bona fide liver ECs after irradiation (Fig. Claim your business to immediately update business information, track page views, and more! I.3) Communication. Brief introductions into these research areas: Photonic Materials & Devices ; Quantum Optics & Spectroscopy; Biomedical Photonics; Optical Systems ; Solar Energy . Donor chimerism in the bone marrow of recipient animals was determined after sacrificing animals. — Sicherung Spritzbeton bis d= 30cm = 2 500 m2. Afterwards, the skins of the two mice were sewn together. Then, the left lateral lobe and the median lobe were ligated with 4–0 silk sutures and resected. Create New Account. Location & Hours. In brief, the read parts derived from RNA molecules were aligned to the mouse reference genome (mm10) using splicing-aware aligner STAR (version 2.5.1b) (Dobin and Gingeras, 2015); the read parts containing the information on cell indexes and unique molecular identifiers (UMI) were used to distinguish cells and to count RNA molecules, respectively. Values of P < 0.05 were considered statistically significant. All stainings were performed in 5% FCS (in PBS) on ice for 30 min with optimal dilutions of commercially prepared antibodies. Information about authorised persons and opening procedure: Official name: Vergabekammer Baden-Württemberg, RP Karlsruhe – Referat 15, Official name: Regierungspräsidium Karlsruhe, Referat 42. 1 A; and Video 1), suggesting that bone marrow–recruited cells had incorporated into the liver vasculature independent of the PHx-induced regenerative burst, possibly as a result of irradiation-induced vascular damage. 3 D). WE ARE WHERE THE FORESTS ARE Landratsamt Alb-Donau-Kreis Schillerstrasse 30 . Embryonic tissue was used for genotyping. However, a subsequent randomized controlled trial involving 58 patients with decompensated alcoholic liver disease resulted in no additional benefit from autologous BMDMC infusion combined with standard medical therapy as compared with standard medical therapy alone (Spahr et al., 2013). Regierungspräsidium Karlsruhe Karlsruhe, Baden-Württemberg, Germany 3 weeks ago Be among the first 25 applicants. The animals were rested for 1 mo after tamoxifen application. Your job seeking activity is only visible to you. The potential therapeutic manipulation of visceral organs has been complicated by the carbohydrate surface layer—here, called the mesopolysaccharide (MPS)—that … Then the percentage of YFP+/GFP+ cells within the mononuclear population was determined. Continue. All animal studies were approved by the Regierungspräsidium (Karlsruhe, Germany). The adult liver is the only organ that can completely regenerate after injury or partial resection. All procedures were performed according to the Guide of the Care and Use of Laboratory Animals published by the National Academy of Sciences and were approved by the local authorities (Regierungspräsidium Karlsruhe AZ: 35-9185.81/G—19/12, 35-9185.81/G—229/12). Author Contributions . All experiments were approved by the regional animal welfare authority (Regierungspräsidium Karlsruhe, animal protocols: G127/16; DKFZ383) and were in accordance with the Federation for Laboratory Animal Science Associations (FELASA, category B) and Society for Laboratory Animal Science (GV-Solas, standard guidelines) and with the Guide for the Care and Use of Laboratory Animals published by the … Fig. Little is known about the mechanisms converting psychosocial stress into cellular dysfunction. Male mice (8–10 wk of age) were used in this study unless otherwise indicated. Cells were gated based on size and granularity. M. Singhal, X. Liu, H.G. B was therefore ordered to leave that Member State within one month of the entry into force … S3 A). 76131 Karlsruhe. 2 A). (A) Experimental outline of PHx-induced liver regeneration in irradiation-conditioned GFP+ bone marrow–transplanted mice in the absence or presence of a liver shield. 2 C). BRIEF OVERVIEW OF FOREST MANAGEMENT These two certificates have been awarded to the state operated ForstBW for their sustainable forestry management practices. After sedation with azaperon (5 mg/kg IM; Elanco, … Animals were housed in the same room with a 12-h/12-h light/dark cycle in Makrolon Type II (360 cm 2 ) cages with standard bedding, either alone or in groups, and had ad libitum access to standard chow and water. Recommended Reviews. Publications Lines of Disconnection: The Penguin London Tube Anniversary Series ... this article analyses how Edwidge Danticat’s Claire of the Sea Light and Junot Díaz’s The Brief Wondrous Life of Oscar Wao re-write the stereotype of the paradisiacal and picturesque islands. Regierungspräsidium Karlsruhe Délivrance le oct. 2014. (B) Mice were irradiated with or without liver shield. S3 B). We have in the present study employed multiple irradiation-based myeloablative and nonmyeloablative mouse models that allowed us to unambiguously evaluate the contribution of different cellular sources to the regenerating liver vasculature following two-thirds PHx. Genomic DNA was extracted from tail biopsies using the mouse direct PCR Lysis Reagent Kit (Viagen Biotech, Los Angeles, CA) supplemented with proteinase K (Sigma-Aldrich) according to the manufacturer's instructions. H.G. Dead cells were excluded by staining with Sytox Blue (Invitrogen). GSE116377. We detected that you disabled the JavaScript feature in your browser. VECad-CreERT2xRosa26-YFPfl/fl mice with labeled ECs were intraperitoneally injected with either peanut oil alone or in combination with CCl4 (0.7 µl/g body weight) three times a week for a duration of 4 wk (Scholten et al., 2015). Bone marrow chimeric mice (Fig. We hypothesized that if BMDMCs contribute to the regenerating vasculature, then there should be GFP+ ECs incorporated into the regenerated liver in Para-WT mice after PHx. This work was supported by grants from the Deutsche Forschungsgemeinschaft for projects SFB-873, “Maintenance and differentiation of stem cells in development and disease” (TP B6 to H.G. Zoomed-in images are shown at the bottom. Stay up to date with the best and authentic source of News online. Is this your business? This remarkable feature has led to the development of innovative therapeutic strategies: partial hepatectomy (PHx) for patients with early-stage resectable hepatocellular carcinoma, and split or living donor liver transplantation for patients with end-stage liver disease (Clavien et al., 2007; Michalopoulos, 2007, 2017). Information about authorised persons and opening procedure: Keine Bieter zugelassen. Video 1 illustrates three-dimensional reconstruction of the liver vasculature of the sham-operated mouse 1 mo after irradiation-conditioned bone marrow transplantation.

Schachspiel Holz Klappbar, Radweg Altmühltal übernachtungen, Kronen Zeitung Steiermark Heute, Ab Welchem Bmi Lebensgefahr, Tum Deutschlandstipendium Auftaktveranstaltung, Döner Tellingstedt Telefonnummer, Laura Schilling Leuphana,

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